Flow Cytometry Lab
Denver Health has recently opened a new Flow Cytometry Laboratory dedicated to research on inflammation in severely injured patients. Our flow cytometer, donated to Denver Health by the family and friends of a grateful patient, analyzes multiple features of individual cells as they flow through a fluid medium. It is currently being used to study changes in the surface antigens on white blood cells following traumatic injury.
Equipment:
2004 Beckman Coulter FC500 Dual Laser (488nm & 650nm) High-speed cell analyzer
What is Flow Cytometry used for?
Flow Cytometry, or so-called FACS-Analyses, is one of the most evolving technologies in micro- and molecular biological analyses. Its unique properties allow the measurement of several characteristics (relative size, granularity, and up to five colors of emitted fluorescence) in parallel and correlated particle by particle. Various uses of flow cytometry include measurements of auto-fluorescent proteins, antigen or ligand density, apoptosis, enzyme activity, DNA and RNA content, membrane potential, cytokine receptors and synthesis, drug uptake and efflux, proliferation, phagocytosis, and viability of whole cells, isolated nuclei, organelles or microorganisms. Changes in cell cycle, intracellular pH, intracellular calcium, intracellular glutathione and oxidative burst can be also detected with these instruments.
Why use Flow Cytometry?
- Thousands to millions of cells can be analyzed
- Statistical information is produced simultaneously
- Flexibility in the acquisition of data
- Ability to reanalyze archived data
- Separate up to four target populations simultaneously
- Data may be transmitted to a stand-alone FCS PC workstation for analysis and/or results printed as user-designed hard copies of publishable quality
Who can utilize Flow Cytometry?
- Research laboratories
- Cell Biologists
- Oncologists
- Hematologists
- Pathologists
Rates:
- Academic: $65/hour
- Commercial: $80/hour